CBFB protein (His tag) (80R-1429)

Purified recombinant Human CBFB protein

Synonyms PEA2 protein, Core-binding factor protein, beta subunit, CBF-beta protein, PEBP2B (polyomavirus enhancer binding protein b) protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of CBFB protein (His tag) (80R-1429)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-182 amino acids: MGSSHHHHHH SSGLVPRGSH MPRVVPDQRS KFENEEFFRK LSRECEIKYT GFRDRPHEER QARFQNACRD GRSEIAFVAT GTNLSLQFFP ASWQGEQRQT PSREYVDLER EAGKVYLKAP MILNGVCVIW KGWIDLQRLD GMGCLEFDEE RAQQEDALAQ QAFEEARRRT REFEDRDRSH REEMEVRVSQ LLAVTGKKTT RP
Expression System E.coli
Purity > 85% pure
Molecular Weight 23.6 kDa(202aa), confirmed by MALDI-TOF
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM MES, pH 6.0, containing 0.1mM PMSF and 10% glycerol.
Concentration 0.5 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance CBFB, also known as PEBP2B, is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e.g., RUNX1) and osteogenesis (e.g., RUNX2). This protein is a non-DNA binding regulatory subunit and it allosterically enhances DNA binding by alpha subunit. Recombinant human CBFB protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

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Images

  • Coomassie Blue stained SDS-PAGE of CBFB protein (His tag) (80R-1429) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $255.00
Size: 50 ug
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