CBP antibody (10R-1204)

Mouse monoclonal CBP antibody

Synonyms Monoclonal CBP antibody, Anti-CBP antibody, CREB binding protein antibody
Specificity Human CBP
Cross Reactivity Human, Mouse. Other species not studied
Applications Dot Blot, ICC, IP, WB
Immunogen CBP antibody was raised in mouse using recombinant Creb-Binding Protein (Cbp)

Images

Immunofluorescent staining using CBP antibody (10R-1204)

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were stained with anti-CBP antibody.

Specifications

Host Mouse
Clone CBP5I001
Isotype IgG1
Method of Purification CBP antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer CBP antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations WB: 0.2-2 ug/ml IP: 100-500 ug/sample, ICC: 2-100 ug/ml

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance Acetyltransferase enzyme. Acetylates histones, giving a specific tag for transcriptional activation. Also acetylates non-histone proteins, like NCOA3 coactivator. Mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. CREBBP, as coactivator, augments the activity of phosphorylated CREB to activate transcription of cAMP-responsive genes.

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Images

  • Immunofluorescent staining using CBP antibody (10R-1204) | Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were stained with anti-CBP antibody.
  • Western Blot analysis of immunized recombinant protein using CBP antibody (10R-1204)

Availability: In stock

Price: $190.00
Size: 50 ug
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