CCNA2 antibody (10R-1588)

Mouse monoclonal CCNA2 antibody

Synonyms Monoclonal CCNA2 antibody, Anti-CCNA2 antibody, CCNA antibody, CCNA2, CCNA-2, CCNA 2 antibody, CCNA 2, CCN1 antibody, CCNA-2 antibody, Cyclin A2 antibody
Specificity Human CCNA2
Cross Reactivity Human, Mouse, Rat. Other species not studied
Applications Dot Blot, ICC, WB
Immunogen CCNA2 antibody was raised in mouse using recombinant Human Cyclin A2 (Ccna2)

Images

Immunofluorescent staining using CCNA2 antibody (10R-1588)

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-CCNA2 antibody.

Specifications

Host Mouse
Clone 2112C2a
Isotype IgG1
Method of Purification CCNA2 antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer CCNA2 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3 ).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations WB: 0.2-2 ug/ml

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions.

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Images

  • Immunofluorescent staining using CCNA2 antibody (10R-1588) | Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-CCNA2 antibody.
  • Western Blot analysis of immunized recombinant protein using CCNA2 antibody (10R-1588)

Availability: In stock

Price: $190.00
Size: 50 ug
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