CCNB1 antibody (10R-2271)

Mouse monoclonal CCNB1 antibody

Synonyms Monoclonal CCNB1 antibody, Anti-CCNB1 antibody, Cyclin B1 antibody, CCNB antibody, CCNB1, CCNB 1, CCNB-1, CCNB 1 antibody, CCNB-1 antibody
Cross Reactivity Human
Applications ELISA, IF, WB
Immunogen CCNB1 antibody was raised in Mouse using a purified recombinant fragment of human CCNB1 expressed in E. coli as the immunogen.

Images

Flow Cytometric assay using Western Blot analysis using CCNB1 antibody (10R-2271) (CCNB1 antibody)

FC analysis of Hela cells using CCNB1 antibody (green) and negative control (purple).

Specifications

Host Mouse
Clone 5G6
Isotype IgG1
Form & Buffer Supplied as a liquid in ascitic fluid containing 0.03% sodium azide.
Concentration 1.0 mg/ml

Usage & Assay Information

Usage Recommendations ELISA: 1:10,000, IF: 1:200-1:1000, WB: 1:500-1:2000

Storage & Safety

Storage Store at 4 deg C for short term storage. Store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance CCNB1 is a regulatory protein involved in mitosis. CCNB1 complexes with p34(Cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. CCNB1 has higher expression in tumor tissues.

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Images

  • Flow Cytometric assay using Western Blot analysis using CCNB1 antibody (10R-2271) (CCNB1 antibody) | FC analysis of Hela cells using CCNB1 antibody (green) and negative control (purple).
  • Western Blot analysis using CCNB1 antibody (10R-2271) | Western Blot showing CCNB1 antibody used against Hela (1) and PC-12 (2) cell lysate.
  • Immunofluorescent staining using Western Blot analysis using CCNB1 antibody (10R-2271) (CCNB1 antibody) | Immunofluorescence analysis of Hela cells using CCNB1 antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Availability: In stock

Price: $345.00
Size: 100 ul
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