CD144 antibody (20R-CR019)

Rabbit polyclonal CD144 antibody

Synonyms Polyclonal CD144 antibody, Anti-CD144 antibody, CD 144, CD-144, CD-144 antibody, VE-Cadherin antibody, CD144, CD 144 antibody
Specificity Human
Cross Reactivity Bovine, mouse, rat
Applications ELISA, FC, IHC-F, WB
Immunogen CD144 antibody was raised in rabbit using recombinant protein fragments corresponding to amino acids 1-258 of VE-cadherin as the immunogen.

Images

Flow cytometric assay using CD144 antibody (20R-CR019)

Recognition of native VE-cadherin by anti-VE-Cad. Specificity of the anti-fragment antibody anti-VE-Cad was examined by flow cytometry using VE-cadherin-expressing CHO cells( A ) (hatched surface, VE-cadherin transfectant; open surface, CHO control), by immunofluorescence staining of fixed confluent endothelial cell monolayers (B ), or by Western blot using endothelial cell lysates ( C).

Specifications

Host Rabbit
Method of Purification CD144 antibody was purified by affinity chromatography.
Form & Buffer Purified IgG supplied in PBS.
Concentration Batch dependent - please inquire should you have specific requirements

Usage & Assay Information

Usage Recommendations Optimal conditions to be determined by end user

Storage & Safety

Storage Aliquot and store at -20 deg C. Avoid repeated Freeze/Thaw cycles

General Information

Biological Significance VE-Cadherin is a 120 kDa member of the type II Cadherin family, characterized by the presence of 5 extracellular cadherin domains (ECD), and anchored to the actin cytoskeleton through their cytoplasmic tail. VE-Cadherin mediates homophilic adhesion between neighbouring endothelial cells and is localized within specialized structures at cell-cell contacts, called adherens junctions.

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Images

  • Flow cytometric assay using CD144 antibody (20R-CR019) | Recognition of native VE-cadherin by anti-VE-Cad. Specificity of the anti-fragment antibody anti-VE-Cad was examined by flow cytometry using VE-cadherin-expressing CHO cells( A ) (hatched surface, VE-cadherin transfectant; open surface, CHO control), by immunofluorescence staining of fixed confluent endothelial cell monolayers (B ), or by Western blot using endothelial cell lysates ( C).
  • Western blot analysis using CD144 antibody (20R-CR019) | Immunoblot analysis of VE-cadherin and catenin expression in endothelial cells exposed to anti-Cad2 antibody. A, total cell lysates of untreated cells (lanes 1 and 4) and of cells treated with anti-Cad2 antibody for 2 h (lanes 2 and5) or 4 h (lanes 3 and6) were electrophoresed and transferred to nitrocellulose membranes before probing with anti-VE-Cad, anti-alpha-catenin, anti-beta-catenin, and anti-plakoglobin (plako) antibodies.Lanes 4–6 correspond to cells treated with cycloheximide for 4 h prior to the addition of anti-VE-Cad.B, shown are the results from the quantification of the amounts of VE-cadherin and catenins. The amounts of VE-cadherin (panel a), alpha-catenin (panel b), beta-catenin (panel c), and plakoglobin (panel d) were estimated, using image analysis software, from the ECL bands of the Western blots described for A. Shaded bars, without cycloheximide; white bars, with cycloheximide. The relative amounts of prote

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Price: $625.00
Size: 100 ug
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