CD247 antibody (10R-2171)

Mouse monoclonal CD247 antibody

Synonyms Monoclonal CD247 antibody, Anti-CD247 antibody, CD-247 antibody, CD247, CD-247, Cluster of Differentiation 247 antibody, T-cell surface glycoprotein CD3 zeta chain antibody, CD 247 antibody, Cluster of differentiation 247 antibody, CD 247, T-cell receptor T3 zeta chain antibody
Cross Reactivity Human
Applications ELISA, FC, IF, WB
Immunogen CD247 antibody was raised in Mouse using a purified recombinant fragment of human CD247 expressed in E. coli as the immunogen.

Images

Flow Cytometric assay using Western Blot analysis using CD247 antibody (10R-2171) (CD247 antibody)

FC analysis of Jurkat cells using anti-CD247 antibody (green) and negative control (purple).

Specifications

Host Mouse
Clone 4B10
Isotype IgG1
Form & Buffer Supplied as a liquid in ascitic fluid containing 0.03% sodium azide.
Concentration 1.0 mg/ml

Usage & Assay Information

Usage Recommendations ELISA: 1:10,000, FC: 1:200-1:400, IF: 1:200-1:1000, WB: 1:500-1:2000

Storage & Safety

Storage Store at 4 deg C for short term storage. Store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance CD247, together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response.

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Images

  • Flow Cytometric assay using Western Blot analysis using CD247 antibody (10R-2171) (CD247 antibody) | FC analysis of Jurkat cells using anti-CD247 antibody (green) and negative control (purple).
  • Western Blot analysis using CD247 antibody (10R-2171) | Western Blot showing CD247 antibody used against CD247 (AA: 52-164)-hIgGFc transfected HEK293 cell lysate.
  • Immunohistochemical staining using CD247 antibody (10R-2171) | Immunohistochemical analysis of paraffin-embedded human Thymus tissues using anti-CD247 antibody
  • Immunofluorescent staining using Western Blot analysis using CD247 antibody (10R-2171) (CD247 antibody) | Immunofluorescence analysis of K562 cells using anti-CD247 antibody (green). Blue: DRAQ5 fluorescent DNA dye.

Availability: In stock

Price: $345.00
Size: 100 ul
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