CD274 antibody (10R-8058)

Rat monoclonal CD274 antibody

Synonyms Monoclonal CD274 antibody, Anti-CD274 antibody, PDCD1L1 antibody, PDCD1LG1 antibody, PDL1 antibody, CD 274 antibody, CD-274 antibody, CD274, CD-274, CD274, B7-H antibody, B7H1 antibody, PD-L1 antibody
Applications FC, IHC-F, IP
Immunogen CD274 antibody was raised inRat using Mouse CD274 transfected L5178Y cells as the immunogen

Images

B7 family ligands and CD28 family receptors

Specifications

Host Rat
Clone MIH6
Isotype IgG2a
Method of Purification CD274 antibody was purified by Protein G affinity chromatography
Form & Buffer Supplied in PBS buffer with 0.09% Sodium Azide (NaN3) added as a preservative
Concentration 1.0 mg/ml

Usage & Assay Information

Usage Recommendations FC, IHC-F, IP

Storage & Safety

Storage Store at 4 deg C for short term storage or aliquot and freeze at -20 deg C for long term storage

General Information

Product Use Monoclonal CD274 antibody specific for Human CD274 for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation.
Biological Significance CD274 is a transmembrane protein that has been speculated to play a major role in suppressing the immune system during particular events such as pregnancy, tissue allografts, autoimmune disease and other disease states such as hepatitis. Normally the immune system reacts to foreign antigens where there is some accumulation in the lymph nodes or spleen which triggers a proliferation of antigen-specific CD8+ T cell. The formation of CD274 receptor / PD-L1 or B7.1 receptor /PD-L1 ligand complex transmits an inhibitory signal which reduces the proliferation of these CD8+ T cells at the lymph nodes and supplementary to that CD274 is also able to control the accumulation of foreign antigen specific T cells in the lymph nodes through apoptosis which is further mediated by a lower regulation of the gene Bcl-2.
Expression CD274 is highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes. CD274 is a Single-pass type I membrane protein and a member of the BTN/MOG group in the immunoglobulin superfamily.
Implications in Disease Upregulation of CD274 may allow cancers to evade the host immune system. An analysis of 196 tumor specimens from patients with renal cell carcinoma found that high tumor expression of CD274 was associated with increased tumor aggressiveness and a 4.5-fold increased risk of death. Ovarian cancer patients with higher expression of CD274 had a significantly poorer prognosis than those with lower expression. CD274 expression correlated inversely with intraepithelial CD8+ T-lymphocyte count, suggesting that CD274 on tumor cells may suppress antitumor CD8+ T cells. This has encouraged development of CD274 inhibitors which As of April 2013 have started clinical trials. The effect might be tumor type dependant; a study on patients with non-small cell lung cancer showed that greater PD-L1 protein and mRNA expression is associated with increased local lymphocytic infiltrate and longer survival.
Sequence Information CD274 contains 290 amino acids and has a molecular weight of 33275 Da. It contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Post-Translational Modifications CD274 undergoes glycosylation at Asn35, Asn192, Asn200 and Asn219.
Database Links Entrez Gene: 29126 Human, Omim: 605402 Human, SwissProt: Q9NZQ7 Human, Unigene: 521989 Human

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Images

  • B7 family ligands and CD28 family receptors
  • Flow cytometric assay using CD274 antibody (10R-8058) | Expression of B7-H1 and B7-DC by mouse tumor cell lines. A, Reactivity of CD274 and TY25 with tumor cell lines. T lymphoma cell lines (WR19L, MBL-2, EL-4, and BW5147), B lymphoma cell lines (A20.2J, BAL17, LK35.2, and 2PK-3), macrophage cell lines (J774A.1, RAW264.7, and P388D1), renal carcinoma cell line Renca, erythroleukemia cell line FBL-3, melanoma cell line B16, fibrosarcoma cell line Meth A, and neuroblastoma cell line C1300 were stained with biotinylated control rat IgG2a, CD274, or TY25, followed by PE-labeled streptavidin. The open histograms indicate staining with CD274 or TY25 and the shaded histograms indicate background staining with control rat IgG2a. B, Expression of B7-H1 and B7-DC mRNAs in tumor cell lines. B7-H1 and B7-DC mRNAs were detected by specific RT-PCR amplification of total RNA prepared from the indicated tumor cell lines. Amplification of beta-actin mRNA was used as a control.
  • Immunoprecipitation assay using CD274 antibody (10R-8058) | Immunoprecipitation of B7-H1 and B7-DC Ags with CD274 and TY25antibodiesImmunoprecipitates with control rat IgG2a, CD274, or TY25 from L5178Y, B7-H1/L5178Y, and B7-DC/L5178Y cell lysates were subjected to SDS-PAGE under nonreducing conditions, blotted onto polyvinylidene difluoride membrane, and then detected with biotinylated control rat IgG2a, CD274, or TY25, respectively, followed by the avidin-biotinylated peroxidase complex and ECL. The positions of molecular mass markers are indicated at the left in kilodaltons.
  • Functional assay using CD274 antibody (10R-8058) | Anti-B7-H1 mAb treatment does not induce depletion or apoptosis of CD4+ LPMCs and splenocytes. Mice with established colitis at 6 wk after the transfer of CD4+ CD45RBhigh cells were injected i.p. with either anti-B7-H1 mAb (CD274) or control rat IgG at a dose of 1 mg/body. A, Seventy-two hours after the injection, CD4+ T cell numbers in the spleen and colon were determined. Data are represented as the mean ± SEM of four mice in each group. B, After isolating splenocytes, the number of apoptotic cells was determined by the annexin V-FITC/PI. Data represent one experiment from a mouse treated with anti-B7-H1 mAb or control IgG. C, Data are represented as the mean ± SEM percentage of PI+ annexin V+ (dead) cells and the percentage of PI− annexin V+ (apoptotic) cells from four mice in each group.

Availability: In stock

Price: $195.00
Size: 100 ug
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