CD31 antibody (10R-1928)

Mouse monoclonal CD31 antibody

Synonyms Monoclonal CD31 antibody, Anti-CD31 antibody, Cluster of differentiation 31 antibody, PECAM1 antibody, PECAM-1 antibody, CD31, CD 31, CD-31, CD 31 antibody, CD-31 antibody, Platelet Endothelial Cell Adhesion Molecule antibody, EndoCAM antibody, GPIIA antibody
Cross Reactivity Human
Applications ELISA, IF, IHC, WB
Immunogen CD31 antibody was raised in Mouse using a purified recombinant fragment of human CD31 expressed in E. coli as the immunogen.

Images

Immunofluorescent staining using Western Blot analysis using CD31 antibody (10R-1928) (CD31 antibody)

Immunofluorescence analysis of paraffin-embedded human lung cancer (left) and breast cancer (right) cells using CD31 antibody (green). Blue: DRAQ5 fluorescent DNA dye.

Specifications

Host Mouse
Clone 2F7B2
Isotype IgG1
Form & Buffer Supplied as a liquid in ascitic fluid containing 0.03% sodium azide.
Concentration 1.0 mg/ml

Usage & Assay Information

Usage Recommendations ELISA: 1:10,000, IF: 1:200-1:1000, IHC: 1:200-1:1000, WB: 1:500-1:2000

Storage & Safety

Storage Store at 4 deg C for short term storage. Store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance CD-31 is normally found on endothelial cells, platelets, macrophages and Kupffer cells, granulocytes, T / NK cells, lymphocytes, megakaryocytes, osteoclasts, neutrophils. CD-31 is also expressed in certain tumors, including epithelioid hemangioendothelioma, epithelioid sarcoma-like hemangioendothelioma, other vascular tumors, histiocytic malignancies, and plasmacytomas. It is rarely found in some sarcomas, such as Kaposi's sarcoma, and carcinomas.

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Images

  • Immunofluorescent staining using Western Blot analysis using CD31 antibody (10R-1928) (CD31 antibody) | Immunofluorescence analysis of paraffin-embedded human lung cancer (left) and breast cancer (right) cells using CD31 antibody (green). Blue: DRAQ5 fluorescent DNA dye.
  • Western Blot analysis using CD31 antibody (10R-1928) | Western Blot showing CD31 antibody used against truncated CD31 recombinant protein.
  • Immunohistochemical staining using CD31 antibody (10R-1928) | Immunohistochemical analysis of paraffin-embedded human placenta using CD31 antibody.
  • Immunohistochemical staining using CD31 antibody (10R-1928) | Immunohistochemical analysis of paraffin-embedded human lung cancer (A), lymphonodus tissue (B) and breast cancer (C), showing cytoplasmic localization of vascular endothelial cells using CD31 antibody with DAB staining.

Availability: In stock

Price: $395.00
Size: 100 ul
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