CD45 antibody (10R-2078)

Mouse monoclonal CD45 antibody

Synonyms Monoclonal CD45 antibody, Anti-CD45 antibody, CD-45 antibody, Cluster of differentiation 45 antibody, CD 45, CD-45, H-CAM antibody, HCAM antibody, Pgp1 antibody, CD45, CD 45 antibody, SCID due to PTPRC deficiency antibody, LCA antibody, Protein tyrosine phosphatase receptor type c polypeptide antibody, B220 antibody, receptor type antibody, T200 glycoprotein antibody, protein tyrosine phosphatase antibody, L CA antibody, Leukocyte common antigen antibody
Cross Reactivity Human
Applications ELISA, IHC, WB
Immunogen CD45 antibody was raised in Mouse using a purified recombinant fragment of CD45 expressed in E. coli as the immunogen.

Images

Immunohistochemical staining using CD45 antibody (10R-2078)

Immunohistochemical analysis of paraffin-embedded human lymph node tissue,showing membrane and cytoplasmic localization with DAB staining using CD45 antibody.

Specifications

Host Mouse
Clone 4A8A4C7A2
Isotype IgG1
Form & Buffer Supplied as a liquid in ascitic fluid containing 0.03% sodium azide.
Concentration 1.0 mg/ml

Usage & Assay Information

Usage Recommendations ELISA: 1:10,000, IHC: 1:200-1:1000, WB: 1:500-1:2000

Storage & Safety

Storage Store at 4 deg C for short term storage. Store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance CD45 a receptor type protein tyrosine phosphatase protein. Contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. The first PTPAse domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Specifically expressed in hematopoietic cells. An essential regulator of T- and B-cell antigen receptor signaling.

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Images

  • Immunohistochemical staining using CD45 antibody (10R-2078) | Immunohistochemical analysis of paraffin-embedded human lymph node tissue,showing membrane and cytoplasmic localization with DAB staining using CD45 antibody.
  • Western Blot analysis using CD45 antibody (10R-2078) | Western Blot showing anti-CD45 monoclonal antibody used against truncated CD45 recombinant protein (1).

Availability: In stock

Price: $345.00
Size: 100 ul
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