CD71 antibody (10R-6435)
Rat monoclonal CD71 antibody
|Synonyms||Monoclonal CD71 antibody, Anti-CD71 antibody, CD-71, TFRC antibody, TRFR antibody, TFR antibody, CD71, TFR1 antibody, CD 71 antibody, CD-71 antibody, CD 71|
|Applications||FC, Functional Assay, IHC|
|Assay Information||A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest.|
Flow Cytometric analysis using CD71 antibody (10R-6435)
Surface staining of mouse bone marrow with CD71 antibody (PE) and CD45R antibody (FITC). Cells in the lymphoid population were used for analysis.
|Method of Purification||CD71 antibody was purified by affinity chromatography.|
|Form & Buffer||Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.|
Usage & Assay Information
|Usage Recommendations||FC: = 1 ug/test|
Storage & Safety
|Storage||Store at 2-8 deg C. Do not freeze. Protect from light.|
|Biological Significance||The R17217 monoclonal antibody reacts with mouse CD71, a 170-180 kDa type II transmembrane protein. CD71, the transferrin receptor, exists as a homodimer on the cell surface and is essential for cellular growth. CD71 is expressed by immature proliferating cells and at low levels on resting mature lymphocytes. Antigen or mitogen stimulation of T and B cells upregulates the expression of CD71.|
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