CD73 antibody (10R-1067)

Mouse monoclonal CD73 antibody

Synonyms Monoclonal CD73 antibody, Anti-CD73 antibody, 5'-nucleotidase ecto (CD73) antibody, CD 73, eNT antibody, 5' nucleotidase antibody, CD-73, ecto antibody, CD73, CD73 antibody, CD 73 antibody, eN antibody, 5' nucleotidase (CD73) antibody, CD-73 antibody
Specificity Human
Cross Reactivity To be determined by end-user.
Applications ELISA, WB
Immunogen CD73 antibody was raised in mouse using recombinant human CD73 (27-252aa) purified from E. coli as the immunogen.

Images

Western Blot analysis of CD73 antibody (10R-1067)

Cell lysates of human mesenitrocellulosehymal stem cell (30 ug) were resolved by SDS-PAGE, transferred to nitrocellulose membrane and probed with anti-human CD73 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Host Mouse
Clone 2B6
Isotype IgG2b kappa
Source Anti-human CD73 antibody, clone 2B6, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human CD73 protein.
Method of Purification CD73 antibody was purified by protein-G affinity chromatography
Form & Buffer Supplied as a liquid in PBS, pH 7.4, with 0.1% NaN3.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations WB: 1:1,000-1:2,000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance CD73 is a membrane-bound glycoprotein that functions to hydrolyze extracellular nucleoside monophosphates into bioactive nucleoside intermediates. This enables the uptake of adenosine, inosine, and guanosine into the cell and their subsequent reconversion into ATP and GTP in the purine salvage pathway. The expression of CD73 on neuronal cells has been described during developmental processes and, on lymphocytes, CD73 serves as a maturation marker, being absent from the surface of both immature B and T cells.

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Images

  • Western Blot analysis of CD73 antibody (10R-1067) | Cell lysates of human mesenitrocellulosehymal stem cell (30 ug) were resolved by SDS-PAGE, transferred to nitrocellulose membrane and probed with anti-human CD73 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Availability: In stock

Price: $265.00
Size: 100 ul
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