Cdc2 antibody (10R-2181)

Mouse monoclonal Cdc2 antibody

Synonyms Monoclonal Cdc2 antibody, Anti-Cdc2 antibody, Cell division control protein 2 homolog antibody, Cdk1 antibody, Cdc2, Cdc 2 antibody, Cdc 2, Cdc-2, Cyclin dependent kinase 1 antibody, Cdc-2 antibody
Cross Reactivity Human
Applications ELISA, FC, WB
Immunogen Cdc2 antibody was raised in Mouse using a purified recombinant fragment of Cdc2 expressed in E. coli as the immunogen.

Images

Flow Cytometric assay using Western Blot analysis using Cdc2 antibody (10R-2181) (Cdc2 antibody)

FC analysis of PC-2 cells using CDC2 antibody (green) and negative control (purple).

Specifications

Host Mouse
Clone 8C5A7F10
Isotype IgG1
Form & Buffer Supplied as a liquid in ascitic fluid containing 0.03% sodium azide.
Concentration 1.0 mg/ml

Usage & Assay Information

Usage Recommendations ELISA: 1:10,000, FC: 1:200-1:400, WB: 1:500-1:2000

Storage & Safety

Storage Store at 4 deg C for short term storage. Store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Cdc2 plays a key role in the control of the eukaryotic cell cycle, where it is required for entry into S-phase and mitosis. Cdc2 exists as a complex with both cyclin A and cyclin B. The best characterized of these associations is the Cdc2 p34 cyclin B complex, which is required for the G2 to M phase transition. Activation of Cdc2 is controlled at several steps including cyclin binding and phosphorylation of threonine 161. However, the critical regulatory step in activating Cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Tyr14. Phosphorylation at Tyr15 and inhibition of Cdc2 is carried out by WEE1 and MIK protein kinases while Tyr15 dephosphorylation and activation of Cdc2 is carried out by the Cdc25 phosphatase. The isoform Cdc2deltaT is found in breast cancer tissues. Furthermore, Cdc2/Cdk1 is a key mediator of neuronal cell death in brain development and degeneration.

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Images

  • Flow Cytometric assay using Western Blot analysis using Cdc2 antibody (10R-2181) (Cdc2 antibody) | FC analysis of PC-2 cells using CDC2 antibody (green) and negative control (purple).
  • Western Blot analysis using Cdc2 antibody (10R-2181) | Western Blot showing CDC2 antibody used against Jurkat (1) cell lysate.
  • Immunofluorescent staining using Western Blot analysis using Cdc2 antibody (10R-2181) (Cdc2 antibody) | Immunofluorescence analysis of Hela cells using CDC2 antibody (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Availability: In stock

Price: $345.00
Size: 100 ul
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