CDC25B antibody (70R-10484)

Affinity purified rabbit polyclonal CDC25B antibody

Synonyms Polyclonal CDC25B antibody, Anti-CDC25B antibody, S. pombe antibody, cell division cycle 25 homolog B, CDCB-25 antibody, CDCB 25 antibody, CDCB 25, CDC25B, CDCB-25
Cross Reactivity Human, Pig, Dog
Applications IHC, WB
Immunogen CDC25B antibody was raised in rabbit using the N terminal of CDC25B as the immunogen
Assay Information CDC25B Blocking Peptide, catalog no. 33R-5980, is also available for use as a blocking control in assays to test for specificity of this CDC25B antibody

Images

Immunohistochemical staining using CDC25B antibody (70R-10484)

CDC25B antibody was used for immunohistochemistry at a concentration of 4-8 ug/ml to stain Hepatocytes (arrows) in Human Liver. Magnification is at 400X.

Specifications

Host Rabbit
Form & Buffer Lyophilized powder. Add 50 ul of distilled water. Final antibody concentration is 1 mg/ml in PBS buffer.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations WB: 0.2-1 ug/ml

Storage & Safety

Storage Store at 4 deg C, following reconstitution, aliquot and store at -20 deg C. Avoid repeated freeze-thaw cycles.

General Information

Biological Significance CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined.

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Images

  • Immunohistochemical staining using CDC25B antibody (70R-10484) | CDC25B antibody was used for immunohistochemistry at a concentration of 4-8 ug/ml to stain Hepatocytes (arrows) in Human Liver. Magnification is at 400X.
  • Western Blot analysis using CDC25B antibody (70R-10484) | CDC25B antibody (70R-10484) used at 0.2-1 ug/ml to detect target protein.

Availability: In stock

Price: $345.00
Size: 50 ug
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