CDC25C antibody (10R-1350)

Mouse monoclonal CDC25C antibody

Synonyms Monoclonal CDC25C antibody, Anti-CDC25C antibody, CDC-25C antibody, CDC25C, CDC 25C antibody, CDC 25C, M phase inducer phosphatase 3 antibody, CDC-25C, CDC25 antibody
Specificity Human CDC25C
Cross Reactivity Human. Other species not studied
Applications Dot Blot, ICC
Immunogen CDC25C antibody was raised in mouse using recombinant Human Cell Division Cycle 25 Homolog C (S. Cerevisiae) (Cdc25C)

Images

Immunofluorescent staining using CDC25C antibody (10R-1350)

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-CDC25C antibody.

Specifications

Host Mouse
Clone 2131C2a
Isotype IgG1
Method of Purification CDC25C antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer CDC25C antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3 ).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations ICC: 2-100 ug/ml

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance This gene is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known.

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Images

  • Immunofluorescent staining using CDC25C antibody (10R-1350) | Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-CDC25C antibody.
  • Western Blot analysis of immunized recombinant protein using CDC25C antibody (10R-1350)

Availability: In stock

Price: $190.00
Size: 50 ug
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