CDC27 antibody (10R-1327)

Mouse monoclonal CDC27 antibody

Synonyms Monoclonal CDC27 antibody, Anti-CDC27 antibody, CDC 27 antibody, APC3 antibody, Cell division cycle protein 27 homolog antibody, CDC 27, CDC-27 antibody, HNUC antibody, CDC27Hs antibody, D17S978E antibody, D0S1430E antibody, CDC27, CDC-27
Specificity Human CDC27
Cross Reactivity Human. Other species not studied
Applications Dot Blot
Immunogen CDC27 antibody was raised in mouse using recombinant Human Cell Division Cycle 27 Homolog (S. Cerevisiae) (Cdc27)

Images

Western Blot analysis of immunized recombinant protein using CDC27 antibody (10R-1327)

Specifications

Host Mouse
Clone 2132D9a
Isotype IgG1
Method of Purification CDC27 antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer CDC27 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3 ).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations To be optimized by the end user.

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eucaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins.

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Images

  • Western Blot analysis of immunized recombinant protein using CDC27 antibody (10R-1327)

Availability: In stock

Price: $190.00
Size: 50 ug
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