CDC5L antibody (10R-1324)

Mouse monoclonal CDC5L antibody

Synonyms Monoclonal CDC5L antibody, Anti-CDC5L antibody, dJ319D22.1 antibody, CEF1 antibody, PCDC5RP antibody, hCDC5 antibody, CDC5 cell division cycle 5 -like antibody, KIAA0432 antibody, CDCL 5, CDC5L, CDC-5L antibody, CDC-5L, CDC 5L antibody
Specificity Human CDC5L
Cross Reactivity Human, Mouse, Rat. Other species not studied
Applications Dot Blot, FC, ICC, IP, WB
Immunogen CDC5L antibody was raised in mouse using recombinant Human Cdc5 Cell Division Cycle 5-Like (S. Pombe) (Cdc5L)

Images

Flow Cytometric assay using CDC5L antibody (10R-1324)

HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-CDC5L antibody (shaded) or isotype control (unshaded) followed by Alexa Fluor 488 conjugated goat anti-mouse IgG.

Specifications

Host Mouse
Clone 2136C1a
Isotype IgG1
Method of Purification CDC5L antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer CDC5L antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3 ).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations WB: 0.2-2 ug/ml, IP: 100-500 ug/sample, FC: 0.5-2 ug/sample, ICC: 2-100 ug/ml

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance The protein encoded by this gene shares a significant similarity with Schizosaccharomyces pombe cdc5 gene product, which is a cell cycle regulator important for G2/M transition. This protein has been demonstrated to act as a positive regulator of cell cycle G2/M progression. It was also found to be an essential component of a non-snRNA spliceosome, which contains at least five additional protein factors and is required for the second catalytic step of pre-mRNA splicing.

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Images

  • Flow Cytometric assay using CDC5L antibody (10R-1324) | HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-CDC5L antibody (shaded) or isotype control (unshaded) followed by Alexa Fluor 488 conjugated goat anti-mouse IgG.
  • Western Blot analysis of immunized recombinant protein using CDC5L antibody (10R-1324)
  • Immunofluorescent staining using CDC5L antibody (10R-1324) | Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-CDC5L antibody.

Availability: In stock

Price: $190.00
Size: 50 ug
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