CENPF antibody (10R-1337)

Mouse monoclonal CENPF antibody

Synonyms Monoclonal CENPF antibody, Anti-CENPF antibody, Centromere protein F antibody, hcp-1 antibody, CENF antibody, PRO1779 antibody
Specificity Human CENPF
Cross Reactivity Human. Other species not studied
Applications Dot Blot
Immunogen CENPF antibody was raised in mouse using recombinant Human Centromere Protein F, 350/400Ka (Mitosin) (Cenpf)

Images

Western Blot analysis of immunized recombinant protein using CENPF antibody (10R-1337)

Specifications

Host Mouse
Clone 2161C1a
Isotype IgG1
Method of Purification CENPF antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer CENPF antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3 ).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations To be optimized by the end user.

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance This gene encodes a protein that associates with the centromere-kinetochore complex. The protein is a component of the nuclear matrix during the G2 phase of interphase. In late G2 the protein associates with the kinetochore and maintains this association through early anaphase. It localizes to the spindle midzone and the intracellular bridge in late anaphase and telophase, respectively, and is thought to be subsequently degraded. The localization of this protein suggests that it may play a role in chromosome segregation during mitotis. It is thought to form either a homodimer or heterodimer. Autoantibodies against this protein have been found in patients with cancer or graft versus host disease.

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Images

  • Western Blot analysis of immunized recombinant protein using CENPF antibody (10R-1337)

Availability: In stock

Price: $190.00
Size: 50 ug
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