CENPH protein (His tag) (80R-1963)

Recombinant human CENPH protein

Synonyms Centromere protein H protein, NNF1 protein, CENP H protein, Interphase centromere complex protein 35 protein, PMF1 protein, MIND kinetochore complex component homolog protein, Centromere protein H protein, Kinetochore protein CENP H protein, ICEN35 protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of CENPH protein (His tag) (80R-1963)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 136-247 amino acids: MGSSHHHHHH SSGLVPRGSH MLNKLIMKSQ QESWDLEEKL LDIRKKRLQL KQASESKLLE IQTEKNKQKI DLDSMENSER IKIIRQNLQM EIKITTVIQH VFQNLILGSK VNWAEDPALK EIVLQLEKNV DMM
Expression System E.coli
Purity > 90% pure
Molecular Weight 15.5 kDa (133aa)
Tag/Conjugate His tag
Form & Buffer Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol
Concentration 0.5 mg/ml

Usage & Assay Information

Usage Recommendations CENPH protein (His tag) has been used in SDS PAGE, and may be suitable for use in other assays, to be determined by the end user.

Storage & Safety

Storage Can be stored at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles.

General Information

Biological Significance CENPH, also known as centromere protein H, belongs to the centromere protein H family. This protein is component of the CENPA-NAC (nucleosome-associated) complex that plays a central role in assembly of kinetochore proteins. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Recombinant human CENPH protein, fused to His-tag at N-terminus, was expressed in E.coli and denatured using detergent during a conventional chromatography purification process.

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Images

  • Coomassie Blue stained SDS-PAGE of CENPH protein (His tag) (80R-1963) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $275.00
Size: 100 ug
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