CFL2 protein (His tag) (80R-1943)

Recombinant human CFL2 protein

Synonyms CFL2, Cofilin-2 protein, CFL-2, CFL 2, NEM7 protein, CFL-2 protein, CFL 2 protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of CFL2 protein (His tag) (80R-1943)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-166 amino acids: MGSSHHHHHH SSGLVPRGSH MASGVTVNDE VIKVFNDMKV RKSSTQEEIK KRKKAVLFCL SDDKRQIIVE EAKQILVGDI GDTVEDPYTS FVKLLPLNDC RYALYDATYE TKESKKEDLV FIFWAPESAP LKSKMIYASS KDAIKKKFTG IKHEWQVNGL DDIKDRSTLG EKLGGNVVVS LEGKPL
Expression System E.coli
Purity > 90% pure
Molecular Weight 20.9 kDa (186a), confirmed by MALDI-TOF
Tag/Conjugate His tag
Form & Buffer Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 10% glycerol, 50mM Nacl
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations CFL2 protein (His tag) has been used in SDS PAGE, and may be suitable for use in other assays, to be determined by the end user.

Storage & Safety

Storage Can be stored at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles.

General Information

Biological Significance CFL2 protein belongs to the actin-binding proteins ADF family that contains 1 ADF-H domain. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. Defects in CFL2 are the cause of nemaline myopathy type 7 (NEM7). Recombinant human CFL2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of CFL2 protein (His tag) (80R-1943) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $270.00
Size: 100 ug
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