CHD3 antibody (10R-1944)

Mouse monoclonal CHD3 antibody

Synonyms Monoclonal CHD3 antibody, Anti-CHD3 antibody, Chromodomain-helicase-DNA-binding protein 3 antibody, ZFH antibody, Mi-2a antibody, Mi2-ALPHA antibody, CHD3, CHD 3, CHD-3, CHD 3 antibody, CHD-3 antibody
Cross Reactivity Human, Mouse
Applications ELISA, IF, IHC, WB
Immunogen CHD3 antibody was raised in Mouse using a purified recombinant fragment of human CHD3 expressed in E. coli as the immunogen.

Images

Flow Cytometric assay using Western Blot analysis using CHD3 antibody (10R-1944) (CHD3 antibody)

FC analysis of K562 cells using CHD3 antibody (green) and negative control (purple).

Specifications

Host Mouse
Clone 2G4
Isotype IgG1
Form & Buffer Supplied as a liquid in ascitic fluid containing 0.03% sodium azide.
Concentration 1.0 mg/ml

Usage & Assay Information

Usage Recommendations ELISA: 1:10,000, IF: 1:200-1:1000, IHC: 1:200-1:1000, WB: 1:500-1:2000

Storage & Safety

Storage Store at 4 deg C for short term storage. Store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance CHD3 is a member of the CHD family of proteins which are characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. This protein is one of the components of a histone deacetylase complex referred to as the Mi-2/NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Chromatin remodeling is essential for many processes including transcription. Autoantibodies against this protein are found in a subset of patients with dermatomyositis. Three alternatively spliced transcripts encoding different isoforms have been described.

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Images

  • Flow Cytometric assay using Western Blot analysis using CHD3 antibody (10R-1944) (CHD3 antibody) | FC analysis of K562 cells using CHD3 antibody (green) and negative control (purple).
  • Western Blot analysis using CHD3 antibody (10R-1944) | Western Blot showing CHD3 antibody used against Hela (1), K562 (2), Jurkat (3), NTERA-2 (4), HEK293 (5), Raji (6) cell lysate and mouse brain (7) tissue lysate.
  • Immunohistochemical staining using CHD3 antibody (10R-1944) | Immunohistochemical analysis of paraffin-embedded colon cancer tissues using CHD3 antibody with DAB staining.
  • Immunofluorescent staining using Western Blot analysis using CHD3 antibody (10R-1944) (CHD3 antibody) | Immunofluorescence analysis of Hela cells using CHD3 antibody (green). Blue: DRAQ5 fluorescent DNA dye.

Availability: In stock

Price: $345.00
Size: 100 ug
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