CHEK1 antibody (10R-1343)

Mouse monoclonal CHEK1 antibody

Synonyms Monoclonal CHEK1 antibody, Anti-CHEK1 antibody, CHK1 antibody, CHEK1, CHEK-1, CHEK 1 antibody, Serine/threonine-protein kinase Chk1 antibody, CHEK 1, CHEK-1 antibody
Specificity Human CHEK1
Cross Reactivity Human. Other species not studied
Applications Dot Blot
Immunogen CHEK1 antibody was raised in mouse using recombinant Human Chk1 Checkpoint Homolog (S. Pombe) (Chek1)

Images

Western Blot analysis of immunized recombinant protein using CHEK1 antibody (10R-1343)

Left: CHEK1 staining. Right: Coomassie Blue staining of immunized recombinant protein.

Specifications

Host Mouse
Clone 2178C1a
Isotype IgG1
Method of Purification CHEK1 antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer CHEK1 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3 ).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations To be optimized by the end user.

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance Chk1 encodes a kinase that phosphorylates cdc25, an important phosphatase in cell cycle control, particularly for entry into mitosis. Cdc25, when phosphorylated on serine 216 by chk1 becomes bound by an adaptor protein in the cytoplasm. Therefore it is inhibited from removing the inhibiting phosphate from MPF (mitotic/maturation promoting factor) added by Wee1. Consequently, a cell is prevented from entering mitosis.

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Images

  • Western Blot analysis of immunized recombinant protein using CHEK1 antibody (10R-1343) | Left: CHEK1 staining. Right: Coomassie Blue staining of immunized recombinant protein.

Availability: In stock

Price: $190.00
Size: 50 ug
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