Chicken anti Rat IgG (H + L) (Alk Phos) (43R-1510)

Chicken anti Rat IgG (H + L) secondary antibody (Alk Phos)

Synonyms Chicken anti Rat Immunoglobulin G (H + L) secondary antibody (Alk Phos)
Specificity This antibody reacts with heavy (gamma) chains on rat IgG and light chains on all rat immunoglobulins.
Cross Reactivity No reactivity is observed to non-immunoglobulin rat serum proteins, IgG from human or rabbit or serum proteins from human or rabbit.
Applications User optimized
Immunogen Purified Rat IgG (H&L)


Host Chicken
Source Ig fraction was prepared using eggs obtained from healthy hens of US origin.
Purity > 95% pure
Method of Purification Affinity purified using solid phase Rat IgG (H&L)
Tag/Conjugate Alkaline Phosphatase
Form & Buffer Clear, colorless liquid, containing 30 mM Triethanolamine, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1% (w/v) BSA and 0.05% (w/v) Sodium Azide. Protease/IgG free, pH 7.2.
Concentration 1.5 mg/ml

Usage & Assay Information

Usage Recommendations 1:5000-1:50,000 ELISA/WB, 1:500-1:2000 IHC

Storage & Safety

Storage Store undiluted liquid at 2-8 deg C. For storage at -20 deg C, aliquot and dilute with an equal volume of glycerol to prevent loss of enzymatic activity.

General Information

Biological Significance Immunoglobulin G (IgG) are antibody molecules. Each IgG is composed of four peptide chains - two heavy chains and two light chains. Each IgG has two antigen binding sites. Other Immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer. IgG constitutes 75% of serum immunoglobulins in humans. IgG molecules are synthesized and secreted by plasma B cells.

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Availability: In stock

Price: $165.00
Size: 500 ug
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