CHK2 antibody (10R-1978)

Mouse monoclonal CHK2 antibody

Synonyms Monoclonal CHK2 antibody, Anti-CHK2 antibody, CHK2, CHK 2, CHK-2 antibody, CHK 2 antibody, Checkpoint Homolog 2 antibody, CHK-2
Cross Reactivity Human
Applications ELISA, IF, IHC, WB
Immunogen CHK2 antibody was raised in Mouse using a purified recombinant fragment of human CHK2 (aa481-531) expressed in E. coli as the immunogen.

Images

Immunofluorescent staining using Western Blot analysis using CHK2 antibody (10R-1978) (CHK2 antibody)

Confocal immunofluorescence analysis of Hela cells using CHK2 antibody (green), showing nuclear localization. Red: Actin filaments have been labeled with DY-554 phalloidin.

Specifications

Host Mouse
Clone 1C12B8
Isotype IgG2b
Form & Buffer Supplied as a liquid in ascitic fluid containing 0.03% sodium azide.
Concentration 1.0 mg/ml

Usage & Assay Information

Usage Recommendations ELISA: 1:10,000, IF: 1:200-1:1000, IHC: 1:200-1:1000, WB: 1:500-1:2000

Storage & Safety

Storage Store at 4 deg C for short term storage. Store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance CHK2 is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit Cdc25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage.

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Images

  • Immunofluorescent staining using Western Blot analysis using CHK2 antibody (10R-1978) (CHK2 antibody) | Confocal immunofluorescence analysis of Hela cells using CHK2 antibody (green), showing nuclear localization. Red: Actin filaments have been labeled with DY-554 phalloidin.
  • Western Blot analysis using CHK2 antibody (10R-1978) | Western Blot showing CHK2 antibody used against cell lysate from various cell types.
  • Immunohistochemical staining using CHK2 antibody (10R-1978) | Immunohistochemical analysis of paraffin-embedded human lung carcinoma (A), liver carcinoma (B), breast carcinoma (C) and kiney carcinoma (D), showing nuclear localization with DAB staining using CHK2 antibody.

Availability: In stock

Price: $395.00
Size: 100 ul
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