CHMP2B protein (His tag) (80R-1873)
Purified recombinant CHMP2B protein
|Synonyms||CHMPB 2, VPS2-2 protein, DMT1 protein, DKFZp564O123 protein, CHMP2.5 protein, VPS2B protein, CHMPB-2, CHMPB 2 protein, CHMPB-2 protein, CHMP2B|
Coomassie Blue stained SDS-PAGE of CHMP2B protein (His tag) (80R-1873)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-213 amino acids: MGSSHHHHHH SSGLVPRGSH MASLFKKKTV DDVIKEQNRE LRGTQRAIIR DRAALEKQEK QLELEIKKMA KIGNKEACKV LAKQLVHLRK QKTRTFAVSS KVTSMSTQTK VMNSQMKMAG AMSTTAKTMQ AVNKKMDPQK TLQTMQNFQK ENMKMEMTEE MINDTLDDIF DGSDDEEESQ DIVNQVLDEI GIEISGKMAK APSAARSLPS ASTSKATISD EEIERQLKAL GVD|
|Purity||> 90% pure|
|Molecular Weight||26.1 kDa (233aa), confirmed by MALDI-TOF (molecular weight on SDS-PAGE will appear higher)|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol, 1mM DTT and 0.1M NaCl.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||CHMP2B belongs to the vacuolar sorting protein family. It is a component of the ESCRT-III complex. This complex is crucial for sorting endosomal articles into multivesicular bodies (MVBs), and are also required for the formation of these bodies. CHMP2B widely expressed in brain, heart, skeletal muscle, small intestine, pancreas, lung, placenta and leukocytes. Recombinant human CHMP2B protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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