CIAO1 protein (His tag) (80R-2710)
Purified recombinant CIAO1 protein (His tag)
|Synonyms||Probable cytosolic iron-sulfur protein assembly protein CIAO1 protein, CIA1 protein, WDR39 protein, CIAO1, CIAO 1, CIAO-1, CIAO 1 protein, CIAO-1 protein|
SDS-PAGE analysis of CIAO1 protein (His tag) (80R-2710)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-339 aa: MGSSHHHHHH SSGLVPRGSH MGSMKDSLVL LGRVPAHPDS RCWFLAWNPA GTLLASCGGD RRIRIWGTEG DSWICKSVLS EGHQRTVRKV AWSPCGNYLA SASFDATTCI WKKNQDDFEC VTTLEGHENE VKSVAWAPSG NLLATCSRDK SVWVWEVDEE DEYECVSVLN SHTQDVKHVV WHPSQELLAS ASYDDTVKLY REEEDDWVCC ATLEGHESTV WSLAFDPSGQ RLASCSDDRT VRIWRQYLPG NEQGVACSGS DPSWKCICTL SGFHSRTIYD IAWCQLTGAL ATACGDDAIR VFQEDPNSDP QQPTFSLTAH LHQAHSQDVN CVAWNPKEPG LLASCSDDGE VAFWKYQRPE GL|
|Purity||> 95% pure|
|Molecular Weight||40 kDa|
|Form & Buffer||Supplied in liquid form in 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT.|
Storage & Safety
|Storage||Can be stored at 4 deg C for short terms (1-2 weeks). For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles|
|Biological Significance||CIAO1 is essential component of the cytosolic iron-sulfur (Fe/S) protein assembly machinery. This protein is required for the maturation of extra mitochondrial Fe/S proteins and seems to specifically modulate the trans-activation activity of WT1. As part of the mitotic spindle-associated MMXD complex it may play a role in chromosome segregation. Recombinant human CIAO1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.|
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