CIB2 protein (His tag) (80R-1667)
Purified recombinant Human CIB2 protein
|Synonyms||calcium and integrin binding family member 2 protein, CIB-2, CIB-2 protein, KIP2, CIB 2 protein, CIB 2, CIB2|
Coomassie Blue stained SDS-PAGE of CIB2 protein (His tag) (80R-1667)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-187 amino acids: MGSSHHHHHH SSGLVPRGSH MGNKQTIFTE EQLDNYQDCT FFNKKDILKL HSRFYELAPN LVPMDYRKSP IVHVPMSLII QMPELRENPF KERIVAAFSE DGEGNLTFND FVDMFSVLCE SAPRELKANY AFKIYDFNTD NFICKEDLEL TLARLTKSEL DEEEVVLVCD KVIEEADLDG DGKLGFADFE DMIAKAPDFL STFHIRI|
|Purity||> 90% pure|
|Molecular Weight||23.8 kDa (207aa), confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol, 2mM DTT, 100mM NaCl and 1mM PMSF.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||CIB2 belongs to calcium and integrin-binding family. CIB2 may be a Ca2+-binding regulatory protein that interacts with DNA-dependent protein kinase catalytic subunit (DNA-PKcs). In skeletal muscle, CIB2 colocalizes with the integrin alpha7B subunit at the sarcolemma and at the neuromuscular and myotendinous junctions. CIB2 is closely related to CIB1. Recombinant human CIB2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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