CKMT2 protein (His tag) (80R-2339)
Purified recombinant Human CKMT2 Protein (His tag)
|Synonyms||Creatine kinase S-type mitochondrial protein, CKMT 2 protein, CKMT 2, CKMT2, CKMT-2, CKMT-2 protein, SMTCK protein|
Coomassie Blue stained SDS-PAGE of CKMT2 protein (His tag) (80R-2339)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||MGSSHHHHHH SSGLVPRGSH MGSHMEVREQ PRLFPPSADY PDLRKHNNCM AECLTPAIYA KLRNKVTPNG YTLDQCIQTG VDNPGHPFIK TVGMVAGDEE SYEVFADLFD PVIKLRHNGY DPRVMKHTTD LDASKITQGQ FDEHYVLSSR VRTGRSIRGL SLPPACTRAE RREVENVAIT ALEGLKGDLA GRYYKLSEMT EQDQQRLIDD HFLFDKPVSP LLTCAGMARD WPDARGIWHN YDKTFLIWIN EEDHTRVISM EKGGNMKRVF ERFCRGLKEV ERLIQERGWE FMWNERLGYI LTCPSNLGTG LRAGVHVRIP KLSKDPRFSK ILENLRLQKR GTGGVDTAAV ADVYDISNID RIGRSEVELV QIVIDGVNYL VDCEKKLERG QDIKVPPPLP QFGKK|
|Purity||> 90% pure|
|Molecular Weight||46.1 kDa|
|Form & Buffer||Supplied in liquid form in 20 mM Tris-HCl buffer, pH 8.0, 10% glycerol, 1mM DTT, 100mM NaCl|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1-2 weeks). Aliquot and store at -20 deg C to -70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||CKMT2 belongs to the ATP:guanido phosphotransferase family. SMTCK is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. This enzyme is reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Recombinant human CKMT2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.|
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