Collagen Type IV antibody (biotin) (60R-CR005bt)

Rabbit polyclonal Collagen Type IV antibody (biotin) conjugated

Synonyms Polyclonal Collagen Type IV antibody, Anti-Collagen Type IV antibody, Collagen Of Basement Membrane alpha 1 Chain antibody, Canstatin antibody, Arresten antibody
Specificity Bovine/Human collagen type IV
Cross Reactivity To be determined by end-user
Applications ELISA, IHC, WB
Immunogen Collagen type IV antibody (biotin) was raised in rabbit using collagen type IV from human and bovine placenta as the immunogen.

Specifications

Host Rabbit
Method of Purification Collagen Type IV antibody (biotin) was purified by affinity chromatography.
Tag/Conjugate Biotin
Form & Buffer Lyophilized from 0.125M Na3B4O7, pH 8.0, with 0.075M NaCl, 0.005M EDTA, 10 mg/ml BSA, and 0.01% NaN3. Immunoglobulin and protease free.

Usage & Assay Information

Usage Recommendations ELISA: 1:20,000-1:100,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000
Bioactivity Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues

Storage & Safety

Storage Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Biohazard Information This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

General Information

Biological Significance Collagen is a group of naturally occurring proteins found, in nature, exclusively in animals, especially in the flesh and connective tissues of mammals. It is the main component of connective tissue, and is the most abundant protein in mammals, making up about 25% to 35% of the whole-body protein content. Type-IV collagen is a type of collagen found primarily in the basal lamina. The C-terminus domain is not removed in post-translational processing, and the fibers link head-to-head, rather than in parallel. Also, type-IV lacks the regular glycine in every third residue necessary for the tight, collagen helix.

References
Add a Paper

Philip L. Davies, Nicola C. Maxwell, Sailesh Kotecha, O. Brad Spiller

Monoclonal anti-neutrophil elastase antibody characterisation: Ability to block function, detect free versus serpin-complexed enzyme and stain intracellular granule

Journal of Immunological Methods

Volume: 336 Issue: 2 Page: 175-182 DOI: 10.1016/j.jim.2008.04.010

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Availability: In stock

Price: $495.00
Size: 100 ug
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