CPE protein (His tag) (80R-2657)

Purified recombinant Human CPE protein (His tag)

Synonyms CPH protein, Carboxypeptidase E protein, Carboxypeptidase H protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

SDS-PAGE analysis of CPE protein (His tag) (80R-2657)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 43-476 aa: MGSSHHHHHH SSGLVPRGSH MGSLQQEDGI SFEYHRYPEL REALVSVWLQ CTAISRIYTV GRSFEGRELL VIELSDNPGV HEPGEPEFKY IGNMHGNEAV GRELLIFLAQ YLCNEYQKGN ETIVNLIHST RIHIMPSLNP DGFEKAASQP GELKDWFVGR SNAQGIDLNR NFPDLDRIVY VNEKEGGPNN HLLKNMKKIV DQNTKLAPET KAVIHWIMDI PFVLSANLHG GDLVANYPYD ETRSGSAHEY SSSPDDAIFQ SLARAYSSFN PAMSDPNRPP CRKNDDDSSF VDGTTNGGAW YSVPGGMQDF NYLSSNCFEI TVELSCEKFP PEETLKTYWE DNKNSLISYL EQIHRGVKGF VRDLQGNPIA NATISVEGID HDVTSAKDGD YWRLLIPGNY KLTASAPGYL AITKKVAVPY SPAAGVDFEL ESFSERKEEE KEELMEWWKM MSETLNF
Expression System E.coli
Method of Purification CPE protein was purified using Protein G affinity chromatography
Tag/Conjugate His tag
Form & Buffer Supplied in liquid form in 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
Concentration 500 ug/ml

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance CPE is a carboxypeptidase that cleaves C-terminal amino acid residues and is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin. It is a peripheral membrane protein. The protein specifically binds regulated secretory pathway proteins, including prohormones, but constitutively secreted proteins. Mutations in CPE are implicated in type II diabetes. Recombinant human CPE protein, fused to His-tag at N-terminus, was expressed in E.coli.

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Images

  • <div><font face=Calibri size=2>SDS-PAGE analysis of CPE protein (His tag) (80R-2657)</font></div>     | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $270.00
Size: 100 ug
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