CPSF4 protein (His tag) (80R-2727)

Purified recombinant CPSF4 protein (His tag)

Synonyms cleavage and polyadenylation specific factor 4 30kDa protein, CPSF30 protein, NAR protein, NEB1 protein, CPSF4, CPSF 4, CPSF-4, CPSF 4 protein, CPSF-4 protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

SDS-PAGE analysis of CPSF4 protein (His tag) (80R-2727)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-244 aa: MGSSHHHHHH SSGLVPRGSH MGSMQEIIAS VDHIKFDLEI AVEQQLGAQP LPFPGMDKSG AAVCEFFLKA ACGKGGMCPF RHISGEKTVV CKHWLRGLCK KGDQCEFLHE YDMTKMPECY FYSKFGECSN KECPFLHIDP ESKIKDCPWY DRGFCKHGPL CRHRHTRRVI CVNYLVGFCP EGPSCKFMHP RFELPMGTTE QPPLPQQTQP PAKQRTPQVI GVMQSQNSSA GNRGPRPLEQ VTCYKCGEKG HYANRCTKGH LAFLSGQ
Expression System E.coli
Purity > 85% pure
Molecular Weight 29.9 kDa
Tag/Conjugate His tag
Form & Buffer Supplied in liquid form in 20mM Tris-HCl buffer (pH 8.0) containing 0.4M UREA, 10% glycerol
Concentration 1 mg/ml

Storage & Safety

Storage Can be stored at 4 deg C for short terms (1-2 weeks). For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles

General Information

Biological Significance Inhibition of the nuclear export of poly(A)-containing mRNAs caused by the influenza A virus NS1 protein requires its effector domain. The NS1 effector domain functionally interacts with the cellular 30 kDa subunit of CPSF4 an essential component of the 3' end processing machinery of cellular pre-mRNAs. In influenza virus-infected cells, the NS1 protein is physically associated with cleavage and polyadenylation specific factor 4, 30kD subunit. Binding of the NS1 protein to the 30 kDa protein in vitro prevents CPSF binding to the RNA substrate and inhibits 3' end cleavage and polyadenylation of host pre-mRNAs. Recombinant human CPSF4 protein, fused to His-tag at N-terminus, was expressed in E.coli.

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Images

  • <div><font face=Calibri size=2>SDS-PAGE analysis of CPSF4 protein (His tag) (80R-2727)</font></div>     | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $295.00
Size: 100 ug
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