CRYGD protein (His tag) (80R-1944)

Recombinant human CRYGD protein

Synonyms CCP protein, CRYG4 protein, Gamma-crystallin D protein, cry-g-D protein, CCA3 protein, CACA protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of CRYGD protein (His tag) (80R-1944)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-174 amino acids: MGSSHHHHHH SSGLVPRGSH MGKITLYEDR GFQGRHYECS SDHPNLQPYL SRCNSARVDS GCWMLYEQPN YSGLQYFLRR GDYADHQQWM GLSDSVRSCR LIPHSGSHRI RLYEREDYRG QMIEFTEDCS CLQDRFRFNE IHSLNVLEGS WVLYELSNYR GRQYLLMPGD YRRYQDWGAT NARVGSLRRV IDFS
Expression System E.coli
Purity > 95% pure
Molecular Weight 22.9 kDa (194a), confirmed by MALDI-TOF
Tag/Conjugate His tag
Form & Buffer Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 5mM DTT, 10% glycerol, 200mM NaCl
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations CRYGD protein (His tag) has been used in SDS PAGE, and may be suitable for use in other assays, to be determined by the end user.

Storage & Safety

Storage Can be stored at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles.

General Information

Biological Significance CRYGD belongs to the beta/gamma-crystallin family. Crystallins are the dominant structural components of the vertebrate eye lens. Mammalian lens crystallins are divided into alpha, beta, and gamma families. Gamma-crystallins have been involved in cataract formation. Defects in CRYGD are a cause of cataract autosomal dominant (ADC), cataract congenital non-nuclear polymorphic autosomal dominant (CCP), cataract congenital cerulean type 3 (CCA3) and cataract crystalline aculeiform (CACA). Recombinant human CRYGD protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of CRYGD protein (His tag) (80R-1944) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $270.00
Size: 100 ug
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