Crystallin alpha B protein (30R-1182)

Purified recombinant Mouse Crystallin alpha B protein

Synonyms Cryab protein, Crya-2 protein, Crya2 protein, , Crystallin alpha B protein
Species Mouse
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of Crystallin alpha B protein (30R-1182)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-175 amino acids: MDIAIHHPWI RRPFFPFHSP SRLFDQFFGE HLLESDLFST ATSLSPFYLR PPSFLRAPSW IDTGLSEMRL EKDRFSVNLD VKHFSPEELK VKVLGDVIEV HGKHEERQDE HGFISREFHR KYRIPADVDP LTITSSLSSD GVLTVNGPRK QVSGPERTIP ITREEKPAVA AAPKK
Expression System E.coli
Purity > 95% pure
Molecular Weight 20 kDa (175aa), confirmed by MALDI-TOF.
Form & Buffer Supplied as a liquid in 20mM Tris, pH 8.0, containing 10% glycerol.
Concentration 1 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Alpha crystallins are composed of two gene products, alpha-A and alpha-B for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP also known as the HSP20). They act as molecular chaperones and hold them in large soluble aggregates. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha-crystallins are an autokinase activity and participation in the intracellular architecture. CRYAB is expressed widely in many tissues and organs. Recombinant CRYAB was expressed in E. coli and purified by using conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of Crystallin alpha B protein (30R-1182) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $255.00
Size: 100 ug
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