CSK antibody (10R-2264)

Mouse monoclonal CSK antibody

Synonyms Monoclonal CSK antibody, Anti-CSK antibody, C-Src Tyrosine Kinase antibody, Carboxy-terminal Src kinase antibody, MGC117393 antibody
Cross Reactivity Human, Mouse, Monkey, Rat
Applications ELISA, IF, WB
Immunogen CSK antibody was raised in Mouse using a purified recombinant fragment of human CSK expressed in E. coli as the immunogen.

Images

Flow Cytometric assay using Western Blot analysis using CSK antibody (10R-2264) (CSK antibody)

FC analysis of HL-60 cells using CSK antibody (green) and negative control (purple).

Specifications

Host Mouse
Clone 5F3
Isotype IgG1
Form & Buffer Supplied as a liquid in ascitic fluid containing 0.03% sodium azide.
Concentration 1.0 mg/ml

Usage & Assay Information

Usage Recommendations ELISA: 1:10,000, IF: 1:200-1:1000, WB: 1:500-1:2000

Storage & Safety

Storage Store at 4 deg C for short term storage. Store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance CSK is a ubiquitously expressed nonreceptor tyrosine kinase that negatively regulates the Src family kinases (SFK) by phosphorylation of the SFK carboxy-terminal tyrosine. Phosphorylated carboxy-terminal tyrosine binds to the SH2 domain of SFK intramolecularly and leads to folding and inactivation of the SFK . This Csk-catalyzed SFK tyrosine phosphorylation is highly specific and exclusive. The SFK carboxy-terminal tyrosine is the only known physiological substrate of Csk.

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Images

  • Flow Cytometric assay using Western Blot analysis using CSK antibody (10R-2264) (CSK antibody) | FC analysis of HL-60 cells using CSK antibody (green) and negative control (purple).
  • Western Blot analysis using CSK antibody (10R-2264) | Western Blot showing CSK antibody used against NIH/3T3 (1),Hela (2),COS7 (3), Jurkat (4), Raw246.7 (5), A549 (6), HL-60 (7) and PC-12 (8) cell lysate.
  • Immunofluorescent staining using Western Blot analysis using CSK antibody (10R-2264) (CSK antibody) | Immunofluorescence analysis of U251 cells using CSK antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Availability: In stock

Price: $345.00
Size: 100 ul
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