CTCF antibody (10R-1548)

Mouse monoclonal CTCF antibody

Synonyms Monoclonal CTCF antibody, Anti-CTCF antibody, CCCTC-binding factor (Zinc finger protein) antibody
Specificity Human CTCF
Cross Reactivity Human. Other species not studied
Applications Dot Blot, ICC
Immunogen CTCF antibody was raised in mouse using recombinant Human Ccctc-Binding Factor (Zinc Finger Protein)

Images

Immunofluorescent staining using CTCF antibody (10R-1548)

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-CTCF antibody.

Specifications

Host Mouse
Clone 252C3a
Isotype IgG1
Method of Purification CTCF antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer CTCF antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3 ).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations ICC: 2-100 ug/ml

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors.

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Images

  • Immunofluorescent staining using CTCF antibody (10R-1548) | Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-CTCF antibody.
  • Western Blot analysis of immunized recombinant protein using CTCF antibody (10R-1548)

Availability: In stock

Price: $190.00
Size: 50 ug
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