CTDSP1 protein (His tag) (80R-1770)
Purified recombinant Human CTDSP1 protein
|Synonyms||NLIIF protein, CTDSP-1, CTDSP 1, NIF3 protein, CTDSP1, polypeptide A phosphatase 1 protein, CTDSP-1 protein, RNA polymerase II protein, Carboxy-terminal domain protein, CTDSP 1 protein, SCP1 protein|
Coomassie Blue stained SDS-PAGE of CTDSP1 protein (His tag) (80R-1770)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-260 amino acids: MGSSHHHHHH SSGLVPRGSH MDSSAVITQI SKEEARGPLR GKGDQKSAAS QKPRSRGILH SLFCCVCRDD GEALPAHSGA PLLVEENGAI PKTPVQYLLP EAKAQDSDKI CVVIDLDETL VHSSFKPVNN ADFIIPVEID GVVHQVYVLK RPHVDEFLQR MGELFECVLF TASLAKYADP VADLLDKWGA FRARLFRESC VFHRGNYVKD LSRLGRDLRR VLILDNSPAS YVFHPDNAVP VASWFDNMSD TELHDLLPFF EQLSRVDDVY SVLRQPRPGS|
|Purity||> 90% pure|
|Molecular Weight||31.2 kDa (280aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 1mM DTT, 20% glycerol and 0.1M NaCl.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||Carboxy-terminal domain, RNA polymerase II, polypeptide A phosphatase 1, also known as CTDSP1, is a class 2C phosphatase with activity dependent on the conserved DxD motif. CTDSP1 preferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residues repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Also it negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation. Recombinant human CTDSP1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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