Cytokeratin 8 +18 antibody (20R-CP004)

Guinea Pig polyclonal Cytokeratin 8 +18 antibody

Synonyms Polyclonal Cytokeratin 8 +18 antibody, Anti-Cytokeratin 8 +18 antibody, Cytokeratin 8 + 18, Cytokeratin 8/18 antibody, Cytokeratin + 1 8, Cytokeratin + 1 -8, Keratin K8 + K18 antibody, Cytokeratin + 1 8 antibody, Cytokeratin + 1 -8 antibody
Specificity Bovine cytokeratin 8/18
Cross Reactivity Bovine, frog, human, mouse, rat, syrian hamster
Applications ELISA, IHC-F, IHC-P, WB
Immunogen Cytokeratin 8/8 antibody was raised in guinea pig using cytokeratin 8/18 filaments, reconstituted from purified bovine cytokeratins 8 and 18 as the immunogen.

Specifications

Host Guinea Pig
Isotype IgG
Purity Stabilized antiserum
Form & Buffer Supplied as whole antiserum with 0.09% NaN3.

Usage & Assay Information

Usage Recommendations IHC-F: 1:100, IHC-P: 1:50, WB: 1:2,000

Storage & Safety

Storage Store at 4 deg C.
Biohazard Information This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

General Information

Biological Significance Cytokeratins are proteins of keratin-containing intermediate filaments found in the intracytoplasmic cytoskeleton of epithelial tissue. Cytokeratin represents an excellent marker for the discrimination of specific subtypes of adenocarcinoma. Keratin, type I cytoskeletal 8 also known as cytokeratin-8 (CK-8) or keratin-8 (K8) is a keratin protein that in human is encoded by the KRT8 gene. It is often paired with keratin 18.

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Application product used in Fresh-frozen OCT embedded tissue, PFA fixed or paraffin embedded tissue, Cell/tissue culture
Usage 1:500 -1:100 for IHC depending on type of tissue, and fixation or 1:1000-1:500 for ICC on cells or prostate organoid/sphere tissues
Comments This is an excellent antibody, and routinely gives me excellent results. I typically use it to analyze prostate lineages in both adult and embryonic tissue. Works very well on fresh-frozen OCT embedded tissue, and well on PFA fixed or paraffin embedded tissue. Additionally it works well in cell culture and tissue culture experiments.
Rating 5

Availability: In stock

Price: $360.00
Size: 100 ul
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