DAAO protein (His tag) (80R-1161)
Purified recombinant Human DAAO protein
|Synonyms||DAAO protein, D-amino acid oxidase DAO1 protein, DAMOX protein, DAO protein, OXDA protein, MGC35381., D-amino acid oxidase protein, EC 22.214.171.124 protein|
Coomassie Blue stained SDS-PAGE of DAAO protein (His tag) (80R-1161)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-347 amino acids: MGSSHHHHHH SSGLVPRGSH MRVVVIGAGV IGLSTALCIH ERYHSVLQPL DIKVYADRFT PLTTTDVAAG LWQPYLSDPN NPQEADWSQQ TFDYLLSHVH SPNAENLGLF LISGYNLFHE AIPDPSWKDT VLGFRKLTPR ELDMFPDYGY GWFHTSLILE GKNYLQWLTE RLTERGVKFF QRKVESFEEV AREGADVIVN CTGVWAGALQ RDPLLQPGRG QIMKVDAPWM KHFILTHDPE RGIYNSPYII PGTQTVTLGG IFQLGNWSEL NNIQDHNTIW EGCCRLEPTL KNARIIGERT GFRPVRPQIR LEREQLRTGP SNTEVIHNYG HGGYGLTIHW GCALEAAKLF GRILEEKKLS RMPPSHL|
|Purity||> 95% pure|
|Molecular Weight||41.6 kDa (367aa), confirmed by MALDI-TOF.|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 20% glycerol, and 1mM DTT.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||D-amino-acid oxidase (DAAO) is a peroxisomal enzyme which uses flavin adenine dinucleotide (FAD) as a cofactor and oxidizes D-amino acids to the corresponding imino acids, producing ammonia and hydrogen peroxide. Its substrates include a wide variety of D-amino acids, but it is inactive on the naturally occurring L-amino acids. It has been suggested that it is involved in acid base balance in the kidney or it could act as a detoxifying agent which removes D-amino acids accumulated during aging. Recombinant human DAAO protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.|
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