DCK protein (His tag) (80R-1500)
Purified recombinant Human DCK protein
|Synonyms||MGC138632 protein, Deoxycytidine kinase, MGC117410 protein|
Coomassie Blue stained SDS-PAGE of DCK protein (His tag) (80R-1500)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-260 amino acids: MGSSHHHHHH SSGLVPRGSH MADPWQECMD YAVTLARQAG EVVCEAIKNE MNVMLKSSPV DLVTATDQKV EKMLISSIKE KYPSHSFIGE ESVAAGEKSI LTDNPTWIID PIDGTTNFVH RFPFVAVSIG FAVNKKIEFG VVYSCVEGKM YTARKGKGAF CNGQKLQVSQ QEDITKSLLV TELGSSRTPE TVRMVLSNME KLFCIPVHGI RSVGTAAVNM CLVATGGADA YYEMGIHCWD VAGAGIIVTE AGGVLMDVTG GPFDLMSRRV IAANNRILAE RIAKEIQVIP LQRDDED|
|Purity||> 90% pure|
|Molecular Weight||34.6 kDa(296aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 7.5, containing 1mM DTT, 0.1mM PMSF, 2mM EDTA, and 10% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||DCK is a key enzyme in the salvage of deoxyribonucleosides and in the activation of clinically relevant nucleoside analogues. This protein is responsible for the 5`-phosphorylation of purine and pyrimidine deoxynucleosides to the corresponding monophosphates using ATP or UTP as phosphate donors. Deficiency of this enzyme activity is associated with resistance to antiviral and anticancer chemotherapeutic agents, whereas increased enzyme activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. Recombinant human DCK protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.|
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