DDB2 antibody (10R-1367)

Mouse monoclonal DDB2 antibody

Synonyms Monoclonal DDB2 antibody, Anti-DDB2 antibody, Damage specific DNA binding protein 2 antibody, DDB2, DDB-2 antibody, DDB-2, DDB 2, DDB 2 antibody, FLJ34321 antibody
Specificity Human DDB2
Cross Reactivity Human. Other species not studied
Applications Dot Blot, ICC, WB
Immunogen DDB2 antibody was raised in mouse using recombinant Human Damage-Specific Dna Binding Protein 2, 48Kda (Ddb2)

Images

Immunofluorescent staining using DDB2 antibody (10R-1367)

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-ddb2 antibody.

Specifications

Host Mouse
Clone 2246C4a
Isotype IgG1
Method of Purification DDB2 antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer DDB2 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3 ).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations WB: 0.2-2 ug/ml, ICC: 2-100 ug/ml

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance The DDB2 gene plays a role in DNA repair by forming with DDB1 the UV-damaged DNA-binding protein complex (UV-DDB). Binds to pyrimidine dimers. Component of the RBX1-CUL4-DDB2 ubiquitin ligase. Required for histone H3 and histone H4 ubiquitination in response to ultraviolet and may be important for subsequent DNA repair.

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Images

  • Immunofluorescent staining using DDB2 antibody (10R-1367) | Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-ddb2 antibody.
  • Western Blot analysis of immunized recombinant protein using DDB2 antibody (10R-1367) | Left: DDB2 staining. Right: Coomassie Blue staining of immunized recombinant protein.

Availability: In stock

Price: $190.00
Size: 50 ug
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