DDT protein (His tag) (80R-1356)

Purified recombinant Human DDT protein

Synonyms D-dopachrome tautomerase protein, , D dopachrome tautomerase protein, D dopachrome decarboxylase protein, Phenylpyruvate tautomerase II protein, D-dopachtome decarboxylase protein, DDCT protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Specifications

Residues 1-118 amino acids: MGSSHHHHHH SSGLVPRGSH MPFLELDTNL PANRVPAGLE KRLCAAAASI LGKPADRVNV TVRPGLAMAL SGSTEPCAQL SISSIGVVGT AEDNRSHSAH FFEFLTKELA LGQDRILIRF FPLESWQIGK IGTVMTFL
Expression System E.coli
Purity > 95% pure
Molecular Weight 14.8 kDa(138aa), confirmed by MALDI-TOF
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20 mM Tris-HCl Buffer, pH 8.0, containing 10% glycerol.
Concentration 1 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance DDT is an enzyme that catayzes the tautomerization of D-dopachrome to give 5,6-dihydroxyindole (DHI). This protein belongs to the family of lyases, specifically the carboxy-lyases, which cleave carbon-carbon bonds. It shares a homologous amino acid sequence (33% identical) with the macrophage migration inhibitory factor (MIF) and possesses similar tautomerase activity. In addition, it may functions a proinflammatory cytokine. Recombinant human DDT, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

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Availability: In stock

Price: $220.00
Size: 100 ug
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