DDX27 antibody (10R-1351)

Mouse monoclonal DDX27 antibody

Synonyms Monoclonal DDX27 antibody, Anti-DDX27 antibody, DDX 27 antibody, DDX27, FLJ20596 antibody, DDX-27 antibody, HSPC259 antibody, dJ686N3.1 antibody, DKFZp667N057 antibody, DDX-27, DDX 27, FLJ22238 antibody, Rrp3p antibody, FLJ12917 antibody, PP3241 antibody, DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 antibody, MGC1018 antibody, RHLP antibody
Specificity Human DDX27
Cross Reactivity Human, Mouse. Other species not studied
Applications Dot Blot, FC, ICC, WB
Immunogen DDX27 antibody was raised in mouse using recombinant Human Dead (Asp-Glu-Ala-Asp) Box Polypeptide 27 (Ddx27)

Images

Flow Cytometric assay using DDX27 antibody (10R-1351)

HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-DDX27 antibody (shaded) or isotype control (unshaded) followed by Alexa Fluor 488 conjugated goat anti-mouse IgG.

Specifications

Host Mouse
Clone 2251C2a
Isotype IgG1
Method of Purification DDX27 antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer DDX27 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3 ).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations WB: 0.2-2 ug/ml, FC: 0.5-2 ug/sample, ICC: 2-100 ug/ml

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, the function of which has not been determined.

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Images

  • Flow Cytometric assay using DDX27 antibody (10R-1351) | HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-DDX27 antibody (shaded) or isotype control (unshaded) followed by Alexa Fluor 488 conjugated goat anti-mouse IgG.
  • Western Blot analysis of immunized recombinant protein using DDX27 antibody (10R-1351)
  • Immunofluorescent staining using DDX27 antibody (10R-1351) | Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-DDX27 antibody.

Availability: In stock

Price: $190.00
Size: 50 ug
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