DDX3X antibody (10R-1397)

Mouse monoclonal DDX3X antibody

Synonyms Monoclonal DDX3X antibody, Anti-DDX3X antibody, DDXX-3 antibody, DDX14 antibody, HLP2 antibody, DDX3 antibody, DDXX-3, DDXX 3, DEAD (Asp-Glu-Ala-Asp) box polypeptide 3 X-linked antibody, DDX3X, DBX antibody, DDXX 3 antibody
Specificity Human DDX3X
Cross Reactivity Human, Mouse, Rat. Other species not studied
Applications Dot Blot, ICC, IP, WB
Immunogen DDX3X antibody was raised in mouse using recombinant Human Dead (Asp-Glu-Ala-Asp) Box Polypeptide 3, X-Linked (Ddx3X)

Images

Immunofluorescent staining using DDX3X antibody (10R-1397)

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-DDX3X antibody.

Specifications

Host Mouse
Clone 2253C5a
Isotype IgG1
Method of Purification DDX3X antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer DDX3X antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3 ).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations WB: 0.2-2 ug/ml, IP: 100-500 ug/sample, ICC: 2-100 ug/ml

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which interacts specifically with hepatitis C virus core protein resulting a change in intracellular location. This gene has a homolog located in the nonrecombining region of the Y chromosome. The protein sequence is 91% identical between this gene and the Y-linked homolog.

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Images

  • Immunofluorescent staining using DDX3X antibody (10R-1397) | Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-DDX3X antibody.
  • Western Blot analysis of immunized recombinant protein using DDX3X antibody (10R-1397)

Availability: In stock

Price: $190.00
Size: 50 ug
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