DDX5 antibody (10R-1420)

Mouse monoclonal DDX5 antibody

Synonyms Monoclonal DDX5 antibody, Anti-DDX5 antibody, HLR1 antibody, DDX 5, DDX5, HUMP68 antibody, G17P1 antibody, p68 antibody, DKFZp686J01190 antibody, DDX-5 antibody, DDX 5 antibody, DDX-5, DEAD (Asp-Glu-Ala-Asp) box polypeptide 5 antibody
Specificity Human DDX5
Cross Reactivity Human, Mouse, Rat. Other species not studied
Applications Dot Blot, ICC, IP, WB
Immunogen DDX5 antibody was raised in mouse using recombinant Human Dead (Asp-Glu-Ala-Asp) Box Polypeptide 5 (Ddx5)

Images

Immunofluorescent staining using DDX5 antibody (10R-1420)

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-DDX5 antibody.

Specifications

Host Mouse
Clone 2257C3a
Isotype IgG1
Method of Purification DDX5 antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer DDX5 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3 ).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations WB: 0.2-2 ug/ml, IP: 100-500 ug/sample, ICC: 2-100 ug/ml

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. This gene consists of 13 exons, and alternatively spliced transcripts containing several intron sequences have been detected, but no isoforms encoded by these transcripts have been identified.

References
Add a Paper

Sorry, but there are no references currently for this product.

Reviews

You need to be logged in to write a review. Please login here

Sorry there are currently no reviews for this product

Images

  • Immunofluorescent staining using DDX5 antibody (10R-1420) | Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-DDX5 antibody.
  • Western Blot analysis of immunized recombinant protein using DDX5 antibody (10R-1420)

Availability: In stock

Price: $190.00
Size: 50 ug
OR
Shipping
View Our Distributors