DFFA protein (His tag) (80R-1185)
Purified recombinant Human DFFA protein
|Synonyms||alpha polypeptide protein, DNA fragmentation factor subunit alpha protein, transcript variant 1 protein, DFFA protein, DFF 45 protein, alpha subunit protein, 45kDa protein, DNA fragmentation factor 45 kDa subunit protein, Dffa DNA fragmentation factor protein, Caspase activated deoxyribonuclease inhibitor short form protein, Caspase-activated deoxyribonuclease inhibitor short form protein, H13 protein, ICAD S protein, DNA fragmentation factor protein, DFF45 protein, DFF35 protein, DNA fragmentation factor protein, DNA fragmentation factor protein, ICAD protein, OTTHUMP00000001904 protein, DNA Fragmentation Factor Alpha Subunit protein, RP23 121D17.3 protein, alpha subunit protein, A330085O09Rik protein, DNA fragmentation factor protein, alpha polypeptide (DFFA) protein, alpha subunit protein, Inhibitor of Caspase Activated DNase protein, DNAation factor 45 kDa subunit protein, FF-45 protein, DFF 1 protein, DFF alpha protein, MGC143066 protein, 45 kD protein, , DNA fragmentation factor subunit alpha protein, 45kDa protein, DFF1 protein, ICAD L protein, OTTHUMP00000001903 protein, Inhibitor of CAD protein|
Coomassie Blue stained SDS-PAGE of DFFA protein (His tag) (80R-1185)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-331 amino acids: MGSSHHHHHH SSGLVPRGSH MEVTGDAGVP ESGEIRTLKP CLLRRNYSRE QHGVAASCLE DLRSKACDIL AIDKSLTPVT LVLAEDGTIV DDDDYFLCLP SNTKFVALAS NEKWAYNNSD GGTAWISQES FDVDETDSGA GLKWKNVARQ LKEDLSSIIL LSEEDLQMLV DAPCSDLAQE LRQSCATVQR LQHTLQQVLD QREEVRQSKQ LLQLYLQALE KEGSLLSKQE ESKAAFGEEV DAVDTGISRE TSSDVALASH ILTALREKQA PELSLSSQDL ELVTKEDPKA LAVALNWDIK KTETVQEACE WELALRLQQT QSLHSLRSIS ASKASPPGDL QNPKRARQDP T|
|Purity||> 90% pure|
|Molecular Weight||38.7 kDa (351aa), confirmed by MALDI-TOF.|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol, and 1mM DTT.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kDa (DFFB) and 45-kDa (DFFA) subunits. DFFA, also known as DNA fragmentation factor subunit alpha, it is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Recombinant DFFA protein was expressed in E. coli and purified by using conventional chromatography techniques.|
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