DFFB antibody (10R-1361)

Mouse monoclonal DFFB antibody

Synonyms Monoclonal DFFB antibody, Anti-DFFB antibody, DFF-40 antibody, DFF2 antibody, DFF40 antibody, DNA fragmentation factor subunit beta antibody, CAD antibody, CPAN antibody
Specificity Human DFFB
Cross Reactivity Human. Other species not studied
Applications Dot Blot
Immunogen DFFB antibody was raised in mouse using recombinant Human Dna Fragmentation Factor, 40Kda, Beta Polypeptide (Caspase-Activated Dnase) (Dffb)


Western Blot analysis of immunized recombinant protein using DFFB antibody (10R-1361)


Host Mouse
Clone 2263C6a
Isotype IgG1
Method of Purification DFFB antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer DFFB antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3 ).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations To be optimized by the end user.

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance DNA fragmentation factor subunit beta is a protein that in humans is encoded by the DFFB gene. Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40kDa (DFFB) and 45kDa (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF.

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  • Western Blot analysis of immunized recombinant protein using DFFB antibody (10R-1361)

Availability: In stock

Price: $190.00
Size: 50 ug
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