DHRS4 protein (His tag) (80R-2068)

Recombinant human DHRS4 protein (His tag)

Synonyms PHCR protein, Dehydrogenase/reductase SDR family member 4 protein, NRDR protein, CR protein, PSCD protein, DHRS-4 protein, SDR25C1 protein, SCAD-SRL protein, DHRS 4, DHRS4, SDR25C2 protein, DHRS-4, SDR-SRL protein, DHRS 4 protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of DHRS4 protein (His tag) (80R-2068)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-278 amino acids: MGSSHHHHHH SSGLVPRGSH MGSHMHKAGL LGLCARAWNS VRMASSGMTR RDPLANKVAL VTASTDGIGF AIARRLAQDG AHVVVSSRKQ QNVDQAVATL QGEGLSVTGT VCHVGKAEDR ERLVATAVKL HGGIDILVSN AAVNPFFGSI MDVTEEVWDK TLDINVKAPA LMTKAVVPEM EKRGGGSVVI VSSIAAFSPS PGFSPYNVSK TALLGLTKTL AIELAPRNIR VNCLAPGLIK TSFSRMLWMD KEKEESMKET LRIRRLGEPE DCAGIVSFLC SEDASYITGE TVVVGGGTPS RL
Expression System E.coli
Purity > 85% pure
Molecular Weight 32.1 kDa (302aa), confirmed by MALDI-TOF
Tag/Conjugate His tag
Form & Buffer Liquid. 20mM Tris-HCl buffer (pH7.5) containing 20% glycerol, 1mM DTT
Concentration 0.25 mg/ml

Usage & Assay Information

Usage Recommendations Optimal conditions to be determined by end user

Storage & Safety

Storage Can be stored at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles.

General Information

Biological Significance DHRS4, also known as dehydrogenase/reductase SDR family member 4, belongs to the short-chain dehydrogenases/reductases (SDR) family. DHRS4 reduces all trans retinal and 9 cis retinal. This protein can also catalyze the oxidation of all trans retinol with NADP as cofactor, but with much lower efficiency. Also, DHRS4 reduces alkyl phenyl ketones and alpha dicarbonyl compounds with aromatic rings, such as pyrimidine 4 aldehyde, 3 benzoylpyridine, 4 benzoylpyridine, menadione and 4 hexanoylpyridine. Recombinant human DHRS4 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

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Images

  • Coomassie Blue stained SDS-PAGE of DHRS4 protein (His tag) (80R-2068) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $270.00
Size: 10 ug
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