DHX38 antibody (10R-1354)

Mouse monoclonal DHX38 antibody

Synonyms Monoclonal DHX38 antibody, Anti-DHX38 antibody, DHX-38 antibody, DHX 38, DHX-38, DHX38, PRP16 antibody, DDX38 antibody, DEAH (Asp-Glu-Ala-His) box polypeptide 38 antibody, KIAA0224 antibody, PRPF16 antibody, DHX 38 antibody
Specificity Human DHX38
Cross Reactivity Human, Mouse, Rat. Other species not studied
Applications Dot Blot, FC, ICC, IP, WB
Immunogen DHX38 antibody was raised in mouse using recombinant Human Deah (Asp-Glu-Ala-His) Box Polypeptide 38 (Dhx38)

Images

Flow Cytometric assay using DHX38 antibody (10R-1354)

HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-DHX38 antibody (shaded) or isotype control (unshaded) followed by Alexa Fluor 488 conjugated goat anti-mouse IgG.

Specifications

Host Mouse
Clone 2271C1a
Isotype IgG1
Method of Purification DHX38 antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer DHX38 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3 ).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations WB: 0.2-2 ug/ml, IP: 100-500 ug/sample, FC: 0.5-2 ug/sample, ICC: 2-100 ug/ml

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a member of the DEAD/H box family of splicing factors. This protein resembles yeast Prp16 more closely than other DEAD/H family members. It is an ATPase and essential for the catalytic step II in pre-mRNA splicing process.

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Images

  • Flow Cytometric assay using DHX38 antibody (10R-1354) | HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-DHX38 antibody (shaded) or isotype control (unshaded) followed by Alexa Fluor 488 conjugated goat anti-mouse IgG.
  • Western Blot analysis of immunized recombinant protein using DHX38 antibody (10R-1354) | Left: DHX38 staining. Right: Coomassie Blue staining of immunized recombinant protein.
  • Immunofluorescent staining using DHX38 antibody (10R-1354) | Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-DHX38 antibody.

Availability: In stock

Price: $190.00
Size: 50 ug
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