DNAJC19 protein (His tag) (80R-1669)
Purified recombinant Human DNAJC19 protein
|Synonyms||DNAJC-19, Pam18 protein, DNAJC-19 protein, DNAJC19, TIM14 protein, TIMM14, DNAJC 19 protein, DNAJC 19, Mitochondrial import inner membrane translocase subunit TIM14 protein|
Coomassie Blue stained SDS-PAGE of DNAJC19 protein (His tag) (80R-1669)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||19-116 amino acids: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMGRY VLQAMKHMEP QVKQVFQSLP KSAFSGGYYR GGFEPKMTKR EAALILGVSP TANKGKIRDA HRRIMLLNHP DKGGSPYIAA KINEAKDLLE GQAKK|
|Purity||> 90% pure|
|Molecular Weight||15.1 kDa (135aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol, 2mM DTT and 0.1M NaCl.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||DNAJC19 is a single-pass membrane protein that contains a J domain and is localized to the inner membrane of the mitochondrion. Expressed ubiquitously, DNAJC19 functions as a component of the mitochondrial DNAJC19 complex, which is responsible for the ATP-dependent translocation of select proteins from the inner mitochondrial membrane to the mitochondrial matrix. Defects in DNAJC19 are the cause of 3-methylglutaconic aciduria type 5 (MGA5), also known as dilated cardiomyopathy with ataxia (DCMA). Recombinant human DNAJC19 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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