Dnak protein (30R-1021)

Purified recombinant E.coli Dnak protein

Synonyms dnaK protein, Heat shock 70 kDa protein protein, 1-384) protein, Co chaperone with DnaJ protein, Chaperone protein dnaK protein, HSP70 protein, Heat shock protein 70 protein, Dnak (N-term protein, ATPase binding domain protein, Chaperone Hsp70 protein,
Species E.coli
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of Dnak protein (30R-1021)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues ATPase binding domain, N-term; 1-384 amino acids: MGKIIGIDLG TTNSCVAIMD GTTPRVLENA EGDRTTPSII AYTQDGETLV GQPAKRQAVT NPQNTLFAIK RLIGRRFQDE EVQRDVSIMP FKIIAADNGD AWVEVKGQKM APPQISAEVL KKMKKTAEDY LGEPVTEAVI TVPAYFNDAQ RQATKDAGRI AGLEVKRIIN EPTAAALAYG LDKGTGNRTI AVYDLGGGTF DISIIEIDEV DGEKTFEVLA TNGDTHLGGE DFDSRLINYL VEEFKKDQGI DLRNDPLAMQ RLKEAAEKAK IELSSAQQTD VNLPYITADA TGPKHMNIKV TRAKLESLVE DLVNRSIEPL KVALQDAGLS VSDIDDVILV GGQTRMPMVQ KKVAEFFGKE PRKDVNPDEA VAIGAAVQGG VLTG
Expression System E.coli
Purity > 95% pure
Molecular Weight 41.6 kDa (384aa)
Form & Buffer Supplied as a liquid in 25mM Tris-HCl, pH 7.5, containing 100 mM NaCl, 5mM DTT, and 10% glycerol.
Concentration 1 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance DNAK, originally identified for its DNA replication by bacteriophage in E. coli is the bacterial hsp70 chaperone. The protein coding region of the substrate binding domain of DNAK (amino acids 385-546) was amplified by PCR and cloned into an E. coli expression vector. The substrate binding domain of DNAK was overexpressed in E. coli and the recombinant protein was purified to apparent homogeneity by using conventional column chromatography techniques. Additional amino acid(Met) is attached at N- terminus.

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Images

  • Coomassie Blue stained SDS-PAGE of Dnak protein (30R-1021) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $180.00
Size: 100 ug
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