Donkey anti Rabbit IgG (H + L) (HRP) (43C-CB1134)

Donkey anti Rabbit IgG (H + L) secondary antibody (HRP)

Synonyms Donkey anti Rabbit Immunoglobulin G (H + L) secondary antibody (HRP)
Specificity Rabbit IgG (H + L)
Cross Reactivity To be determined by end-user
Applications ELISA, IHC, WB
Immunogen Donkey anti-rabbit IgG (H+L) (HRP) was raised in donkey using rabbit IgG whole molecule as the immunogen.

Images

Western blot analysis using Donkey anti Rabbit IgG (H + L) (HRP) (43C-CB1134)

Western Blot of HRP conjugated Donkey anti-Rabbit IgG. Lane 1: Rabbit IgG. Lane 2: none. Load: 50 ng per lane. none. Peroxidase donkey secondary antibody at 1:1,000 for 60 min at RT Block: Blocking buffer for 30 min at RT. Size: 55 kDa, 28 kDa for Rabbit IgG..

Specifications

Host Donkey
Method of Purification Donkey anti Rabbit IgG (H + L) (HRP) was purified by affinity chromatography.
Tag/Conjugate HRP
Form & Buffer Lyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0.01% NaN3. Immunoglobulin and protease free.

Usage & Assay Information

Usage Recommendations ELISA: 1:7,500-1:30,000, IHC: 1:1,000-1:3,000, WB: 1:1,000-1:3,000

Storage & Safety

Storage Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Immunoglobulin G (IgG) are antibody molecules. Each IgG is composed of four peptide chains - two heavy chains ? and two light chains. Each IgG has two antigen binding sites. Other Immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer.

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Images

  • Western blot analysis using Donkey anti Rabbit IgG (H + L) (HRP) (43C-CB1134) | Western Blot of HRP conjugated Donkey anti-Rabbit IgG. Lane 1: Rabbit IgG. Lane 2: none. Load: 50 ng per lane. none. Peroxidase donkey secondary antibody at 1:1,000 for 60 min at RT Block: Blocking buffer for 30 min at RT. Size: 55 kDa, 28 kDa for Rabbit IgG..

Availability: In stock

Price: $325.00
Size: 1 mg
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